Plasmid

Part:BBa_J100253:Design

Designed by: Grace Harper   Group: Campbell M Lab   (2016-04-05)


This is a modification of pClone Cherry (J100251)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1046
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1046
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1046
    Illegal BamHI site found at 899
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1046
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1046
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Make sure the sticky ends aligned with the ligating promoter.


Source

pClone Cherry (J100251)

References